2. Estimation of Amino Nitrogen in Intact Phospholipids by C. H. Lea and D. N. Rhodes
ثبت نشده
چکیده
1. Polyacids interact with albumin in salt-free solutions to prevent coagulation of the protein by heat. Polyphosphates (nucleic acids), polysulphates (heparin and dextran sulphate) and polyearboxylates (alginic acid and bacterial polyglutamic acid) were all effective. Effects on optical rotation of the albumin were also demonstrated. 2. The protecting agent acts by preventing the coagulation of the albumin, although denaturation occurs to some extent. The molecular size of the protecting agent seems of less importance than the number of acid groups in determining the degree of protection. This work was carried out in the laboratories of the Birmingham branch ofthe British Empire Cancer Campaign.
منابع مشابه
Effects of Bioassay and Age on Amino Acid Digestibility and Metabolizable Energy of Soybean, Sunflower and Canola Meals
These experiments were conducted to determine metabolizable energy and amino acid digestibilities of soybean meal, sunflower meal and canola meal in broiler chickens by using titanium oxide as indigestible marker in intact or caecectomized cockerels. Ileal and total tract digestibility of amino acids were measured using broiler chickens, moreover, digestibility were also determined in the intac...
متن کاملGlucuronic Acid Conjugation of Chlorinated and Some Unsaturated Alcohols by J. N. Smith and R. T. Williams
Baer, E. & McArthur, C. S. (1944). J. biol. Chem. 154,451. Chargaff, E., Ziff, M. & Rittenberg, D. (1942). J. biol. Chem. 144, 343. Entenman, C., Taurog, A. & Chaikoff, I. L. (1944). J. biol. Chem. 155, 13. Folch, J. (1942). J. biol. Chem. 146, 35. Fo]ch, J., Schneider, H. A. & Van Slyke, D. D. (1940). J. biol. Chem. 133, xxxiii. Fowden, L. (1951). Biochem. J. 48, 327. Hack,'M. H. (1953). Bioch...
متن کاملUptake and nitrate accumulation affected by partial replacement of nitrate-N with different source of amino acids in spinach and lettuce
As natural plant growth stimulators, amino acids are widely used to improve the yield and quality of crops. Change in enzymes activities of N assimilation (NR, NiR and GS), residual nitrate (NO3-), soluble protein content, and yield of spinach and lettuce plants were investigated under replacing 20% nitrate-N in the nutrient solution by L-glycine and blood meal amino acids. Seeds of the mention...
متن کاملEvaluation of the Aromaticity of a Non-Planar Carbon Nano-Structure by Nucleus-Independent Chemical Shift Criterion: Aromaticity of the Nitrogen- Doped Corannulene
Substitution of two or four carbon atoms by nitrogen in the corannulene molecule as a carbon nanostructure was done and the obtained structures were optimized at MP2/6-31G(d) level of theory. Calculations of the nucleus-independent chemical shift (NICS) were performed to analyze the aromaticity of the corannulene rings and its derivatives upon doping with N at B3LYP/6-31G(d) level of theory. Re...
متن کامل